This article explores the relationship between affectedness and Differential Object Marking (DOM) of indefinite direct objects in Turkish and Uzbek. We argue that the distribution of DOM in the two Turkic languages is determined by the direct objects' specificity and animacy as nominal semantic properties, and affectedness as a verbal semantic property associated with the direct object. We provide original empirical evidence from two forced-choice studies that investigate DOM along the parameters of animacy, affectedness, and telicity, and their interaction with each other. Our findings indicate that affectedness shapes the distribution of DOM in both Turkish and Uzbek, with an interesting variation in how it is instantiated and interacts w...