Photomicrograph of H & E stained brain tissue sections of all groups.

(A) Brain section of normal group showing normal architecture of neuron (thick arrow), neuroglia cells (wave arrow), blood capillaries (circle) as well as neuropil (thin arrow). (B, C, D & E) Brain sections of infected untreated group showing severe degenerative changes with aggregated T. gondii tachyzoites under meninges (cube), neurons with vacuolated apoptotic nucleus (thick arrow) or lost nucleus (wave arrow), apoptotic and vacuolated neuroglia cells (curved arrow), dilated capillaries (circle), gliosis, edema leading to dispersion between neurofibrils (star), and vacuolations along neuropil (arrowhead). (F) Brain section of Spiramycin treated group showing apoptotic vacuolated neurons (thick arrow), apoptotic neuroglia cells (curved arrows), edema (wave arrow), necrotic areas (arrowhead), and dilated capillaries (circle). (G) Brain section of ZnO NPs treated group showing marked improvement with normal appearance of neurons (thick arrow) and blood capillaries (thick arrow). Neuroglia cells detected in regular form (arrowhead) and vacuolated apoptotic one (thin arrow). Edema and vacuolated neuropil (wave arrow) were still observed.