This paper studies the effects of ambiguity on long-run cooperation, by analyzing the infinitely repeated Prisoner’s Dilemma and its application to Cournot’s duopoly model. We show that ambiguity decreases the likelihood of cooperation in the infinitely repeated Prisoner’s Dilemma, regardless the level of optimism. In the economic application, we find that ambiguity is positively related with static equilibrium quantities and negatively related with the probability of sustaining a tacit collusion, i.e. positively related with competition. In fact, the critical discount factor associated with the probability of achieving a collusive equilibrium can be even higher than one for some parametric combinations. Nevertheless, depending on th...