The Current Status of Semen and Oocytes Cryopreservation

Assisted reproductive technologies are critical in the preservation of gametes from endangered species. As a result, cryobanking is critical in reproduction facilities for the gametes conservation of endangered species for future use. Furthermore, cryobanking allows for the preservation of genetic variability through biotechnological reproduction programs. If oocyte cryopreservation is successful, the timing of in vitro maturation and subsequent to in vitro fertilization (IVF) will be possible. Cattle oocytes are very sensitive to cryopreservation due to their complex structure, and they are also very sensitive to chilling, which can harm their viability. During the cryopreservation process, sperm membrane proteins and carbohydrate composition change, sperm membrane structure is disrupted, and sperm viability is reduced. Extenders are frequently required during cryopreservation, for improving sperm cryopreservation technologies and is therefore necessary to have a thorough understanding of the properties of the extenders. Extenders have been enriched with antioxidants such as Glutathione to protect sperm motility and integrity from oxidative damage and the reactive oxygen species produced during cryopreservation can be neutralized using antioxidants