Fig. S2. Determination of CK2 activity using β-casein as phosphorylatable substrate in CK2 knockout C2C12 cells. from Analysis of the phosphoproteome of CK2<i>α</i>^(–/–)/Δ<i>α′</i> C2C12 myoblasts compared to the wild-type cells

Wild-type (WT), CK2α(-/-)/Δα’, CK2α(-/-) and CK2α’(-/-) C2C12 cells were lysed. Cellular CK2 activity was tested in vitro toward β-casein (left panel). Kinase activity is expressed as the percentage (%) of the activity in comparison to the WT cells (means ± SD values, n = 3; *p < 0.05 vs WT cells)