A3B recolocalizes inside the viral replication centers in A12-infected cells.

A549 cells were infected with HAdV-A12, -B3, -C2 or mock control at a MOI = 3. B3- and C2-infected cells were analyzed 20 hours post infection and A12-infected cells were analyzed 24 hours post infection. (A) The A3B protein (green) and the viral replication centers as identified by EdU labelling (red) were imaged by fluorescence microscopy. (B) A3B labelling was quantified within the EdU+ area. Each circle represents a given cell. The yellow-colored circles correspond to the images shown in panel A. P-values were calculated by unpaired t-tests. (C) Colocalization between A3B and EdU labeling in A12-infected cells was estimated using Manders’ coefficients.