Viral replication was attenuated at 12 and 24 h post-infection in MDCK cells and A549 cells with MOI = 0.001 or 0.0001 at 37°C.

<p>Virus titers were quantified by immunofocus assay (FFU/ml). Data represent the means ± SDs of the results determined for triplicate wells. Replication of WT, 7MB_ED, and 7PK_ED were examined in (A) MDCK cells and (B) A549 cells with MOI = 0.001, and in (C) MDCK cells and (D) A549 cells with MOI = 0.0001. Replication of WT, 8PK_ED, 8PK_BP, and 8PK_BP_CP were examined in MDCK cells with (E) MOI = 0.001 and (F) MOI = 0.0001.