Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology

Metalotioneini (MT) pripadaju velikoj grupi proteina male molekulske težine bogatih cisteinom, izražene sposobnosti za vezivanje jona metala, uključenih u procese održavanja homeostaze metalnih jona i detoksifikacije od teških metala. U radu je analizirana struktura dva transkripta gena za MT tipa 3 poreklom iz semena heljde u razviću. Razlike su nađene pre svega u okviru 3’- UTR sekvenci. Nakon analiza sekvenci urađena je analiza ekspresije tokom hipoksije korišćenjem tehnologije Real Tme RT-PCR.Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the developing buckwheat seed cDNA library. Following sequence analyses, expression profiles during flooding stress were monitored by Real Time RT PCR technology