Not all deubiquitinates mediate misfolded membrane toxicity and toxicity is specific to misfolded membrane proteins.

(A) WT, dfm1Δ, and doa4Δ cells containing either GALpr-HMG2-GFP or EV were compared for growth by dilution assay. Each strain was spotted 5-fold dilutions on glucose or galactose-containing plates to drive Hmg2-GFP overexpression, and plates were incubated at 30°C. (B) Dilution assay as in (A) except in dfm1Δ, ubp2Δ, ubp5Δ, miy1Δ, ubp8Δ, miy2Δ, otu2Δ, ubp1Δ, ubp11Δ, ubp7Δ, and ubp3Δ cells. (C) WT, dfm1Δ, ubp6Δ, doa4Δ, ubp9Δ, and ubp14Δ cells containing either GALpr-ΔssCPY*-Myc or EV were compared for growth by dilution assay. Each strain was spotted 5-fold dilutions on glucose or galactose-containing plates to drive Hmg2-GFP overexpression, and plates were incubated at 30°C. Data information: All dilution growth assays were performed in 3 biological and 2 technical replicates (N = 3). (TIF)