Mechanical properties of the hyphal CW and cytoplasm.

(A) Ratio between the radius and the longitudinal shrinkage (elastic strain) during photoablation assay (n = 10 cells). A ratio close to 2 suggests the abscence of major anisotropies in the CW. (B) CW Young’s divided by turgor pressure, measured along mature hyphae (n = 7 at least for each compartments). (C) Turgor pressure measurement using the percentage of plasmolyzed cells (in which the plasma membrane detaches from the CW) as a function of medium osmolarity (n = 17 cells at least for each molarity). Error bars correspond to +/− SD. Results were compared by using a two-tailed Mann–Whitney test. **, P P S1 Data. CW, cell wall. (TIF)