Degron and RNAi knockdowns of ZYG-9 and TAC-1.

(A-D) Time-lapse maximum intensity projection images of live control (-Auxin) and auxin-induced knockdown (+Auxin) of degron-tagged endogenous zyg-9 locus at indicated stages. mCherry::H2b in magenta; GFP::ASPM-1 in teal. (E) Bar graphs quantifying the spindle polarity and chromosome separation defects in control and auxin treated oocytes. (F) RNAi knockdowns using previously reported conditions and endogenous GFP fusion strains [21]. As also reported by others [44], all GFP::TAC-1 signal is lost from spindle microtubules and poles after zyg-9 RNAi, while GFP::ZYG-9 is lost from poles but not spindle microtubules after tac-1(RNAi). Scale bars = 5 μm. (TIF)