Deletion of <i>OSH4</i> rescues hyphal invasive growth in the <i>drs2</i> mutant.

A) Invasive filamentous growth is specifically restored in the drs2 mutant upon deletion of OSH4. The indicated strains, WT (PY4861), osh4/osh4 (osh4, PY3974), osh7/osh7 (osh7, PY5256), drs2/drs2 (drs2, PY3375), drs2/drs2 osh4/osh4 (drs2 osh4, PY5539) and drs2/drs2 osh7/osh7 (drs2 osh7, PY5297), were grown as in Fig 1A and images were taken after 6 days. Similar results were observed in 2 independent experiments. B) Hyphal growth is restored in the drs2 mutant upon deletion of OSH4. Cells from the indicated strains were incubated with serum as in Fig 1B. Bars are 5 μm. The graph represents the percentage of hyphae in the indicated strains, calculated as in Fig 1C. The percentage of hyphae was 82 ± 8%, 80 ± 6% and 76 ± 9% for the wild-type, osh4 and drs2 osh4 cells, respectively. ***, P < 0.0005; ns, not significant.