The RRM domain and phosphorylation sites of MoSrp1 are important for cell wall integrity and stress tolerance.

(A) Colony of the wild-type P131, the ΔMosrp1 mutant srp1ko1, and its one complementation transformant cSRP1, srp1ko1 expressing the MoSrp1 N-terminus (1–90 aa) (cSRP11-90), (1–130 aa) (cSRP11-130), and its C-terminus (91–206 aa) (cSRP191-206), srp1ko1 expressing mutated MoSrp1S117A (M117), mutated MoSrp1S119A (M119), and mutated MoSrp1S117AS119A (M117/119) cultured on CM plates supplemented with different stress agents including 1 M sorbitol, 100 μg/ml CFW, and 200 μg/ml CR at 5 dpi. (B) Colony of the strains in (A) cultured on MM, MM-N, and MM-C plates at 5 dpi. (TIF)