MoSrp1 homologues in Pezizomycotina fungi are functionally similar but divergent from those in other eukaryotes.

(A) Phylogenetic analysis of MoSrp1 and its orthologues from different fungi (red dot): Fusarium graminearum (FgSrp1), Sclerotinia sclerotiorum (SsSrp1), Botrytis cinerea (BcSrp1), Alternaria alternata (AaSrp1), Aspergillus nidulans (AnSrp1), Neurospora crassa (NcSrp1), Puccinia striformis (PsSrp1), Ustilago maydis (UmSrp1), Schizosaccharomyces pombe (Srp1), Pneumocystis carinii (PcSrp1), and Candida albicans (Slr1), plants (yellow dot): Arabidopsis thaliana (AtSc35, AtRS41, and AtRBP1) and Oryza sativa (OsRS2Z32, OsRS2Z33, and OsRS2Z36), animals (blue dot): Drosophila melanogaster (DmSC35, DmSRp55, and DmB52) and Mus musculus (MmSRSF6, MmSRSF10, and MmSRSF12), and human Homo sapiens (HsSRSF6, HsSRSF10, and HsSRSF12). (B) Sequence alignment of MoSrp1 and its counterparts from other fungi including Pezizomycotina (P) FgSrp1, SsSrp1, BcSrp1, AaSrp1, AnSrp1, and NcSrp1, Taphrinamycotina (T) Srp1 and PcSrp1, Saccharomycotina (S) Slr1 and ClSrp1, and Basidiomycota (B) PsSrp1 and UmSrp1. The conserved amino acids among different fungi were highlighted. The RRM domain and RD/E-rich domain of MoSrp1 were underlined. An asterisk marks the SUMOylation site. (C) Colonies formed on OTA plates at 6 dpi by the strains P131, srp1ko1, cSRP1 and srp1ko1 transformants expressing AtRBP1 (AtRBP1/srp1ko1), AtSC35 (AtSC35/srp1ko1), SpSrp2 (SpSrp2/srp1ko1), SpSrp1 (SpSrp1/srp1ko1), FgSrp1 (FgSrp1/srp1ko1), the MoSrp1-SpSrp1 chimeric structures: SpSrp11-130 (the N terminal 130 aa residues of SpSrp1), MoSrp11-60-SpSrp161-130 (the N terminal 60 aa residues of MoSrp1 fused with the 61–130 aa residues of SpSrp1), SpSrp11-60-MoSrp161-130 (the N terminal 60 aa residues of SpSrp1 fused with the 61–130 aa residues of MoSrp1), and SpSrp180K/81I (SpSrp1 mutated with the sumoylation site). (D) Statistics on colony growth of the strains described in (C). (E) Statistics on conidiation of the strains described in (C). The means and standard deviations in (D-E) were calculated based on three independent experiments by measuring three plates for each replicate. Asterisk marks significant difference between P131 and mutant strains using t-test (p (F) Representative disease lesions on the leaves of susceptible barley sprayed with conidia of the strains described in (C).