OSTEOBLAST DIFFERENTIATION IN BONE MARROW STEM CELLS AFTER CALCIUM SULFATE TREATMENT

Calcium Sulfate is a highly biocompatible material largely employed to treating periodontal disease, alveolar bone loss, and maxillary sinus augmentation. It is completely biodegradable, osteoconductive, cheap, easy to handle, and able to support the defect area until bone growth. However, how Calcium Sulfate acts on osteoblast promoting bone formation is poorly understood. To study how Calcium Sulfate can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were compared in Normal Osteoblasts and Bone Marrow Stem Cells, using real time Reverse Transcription-Polymerase Chain Reaction. The obtained results demonstrated that Calcium Sulfate strongly influences the behavior of Bone Marrow Stem Cells in vitro enhancing proliferation, differentiation and deposition of matrix