The present study focused on the bryoflora of watercourses of the Tiber River basin watercourses (Central Italy). A total of 20 bryophyte species, which included 14 mosses and 6 liverworts, were collected at 32 river stations. Most species were recorded at stations of the watercourses’ upper sections, which have rocky substrate and where there is cool and well oxygenated running water, with low trophic load. Only few species, such as Leptodictyum riparium, Cinclidotus fontinaloides and Riccia fluitans, were also found at stations of the middle and lower sections, which are characterized by slow-flowing, turbid, warm and eutrophic waters. Some species are widely distributed, among which Fontinalis antipyretica ssp. antipyretica and Pla...