In vivo and in vitro studies on the effects of mGnRH on oestradiol-17 beta inter-renal production in the female frog, Rana esculenta, during the post-reproductive period.

Plasma oestradiol-17 beta was measured by RIA, in female, Rana esculenta, submitted to hypophysectomy, gonadectomy, or both, and treated with mammalian gonadotropin-releasing hormone (mGnRH), homologous pituitary homogenate, or both, during the post-reproductive period. In addition, the oestradiol-17 beta release was measured in in vitro incubations of ovaries or interrenals treated with mGnRH, pituitary, or both, during the same period. In vivo and in vitro mGnRH and/or pituitary directly stimulated the production of oestradiol-17 beta by the interrenal, but not by ovary, although the stimulatory effects of the pituitary are minor and delayed with respect to those of mGnRH. These results seem to indicate that mGnRH and pituitary, with probably different mechanisms, stimulate the interrenal to produce high levels of oestradiol which is involved in the post-reproductive refractorines