Comparative monitoring by means of diatoms, macroinvertebrates and chemical parameters of an Apennine watercourse of central Italy: The river Tenna.

TheWFD/60/2000/EC establishes the use of different biological indicators to assess the ecological status of rivers, in addition to chemical, physical and bacteriological parameters. At this purpose, the present study concerning the river Tenna, a central Apennine watercourse (Italy), aimed to analyse and discuss the information given by two biological indices: the Extended Biotic Index or E.B.I. that uses benthic macroinvertebrates, in the version adapted to the Italian rivers (I.B.E.), and the diatom-based Eutrophication and/or Pollution Index (EPI-D). Sampling has been carried out twice, in May (deep water conditions) and October 2006 (low water conditions), in ten stations along the hydrographic basin of the river. Chemical parameters have been determined monthly during a year because they present a higher variability than biotic indicators. The correlations between the biological and abiotic data obtained have been statistically analysed by means of the Spearman’s test. The statistical data, in general, showed a direct correlation between the two biotic indices, and an inverse correlation between these and the chemicalphysical and bacteriological parameters. Some of the differences of judgement emerging from the application of the two biotic indices showed a complementary response of the diatoms and macroinvertebrates to various environmental stresses. These results suggest the usefulness of an integrated multidisciplinary approach, in order to obtain a more accurate diagnosis of the health status of the watercourses