Shows a metaphase-arrested oocyte expressing mCherry::tubulin (left) and degron::GFP::ZYG-9 (right), dissected into a control Meiosis medium solution. Corresponds to Fig 2A. No major changes in spindle length or shape occur. Note that the spindle rotates end-on for a portion of the video, but when it rotates back it is clear that the morphology of spindle has not changed. Time elapsed shown in (min):(sec). Scale bar = 5μm. (MOV)</p
Shows a metaphase-arrested klp-18(RNAi) oocyte expressing GFP::ASPM-1, mCherry::tubulin (left) and d...
(A-D) Time-lapse maximum intensity projection images of live control and TS mutant oocytes expressin...
(A) IF imaging of oocyte spindles in the ZYG-9 AID strain in either control or tac-1(RNAi) condition...
Shows a metaphase-arrested klp-18(RNAi) oocyte expressing mCherry::tubulin (left) and degron::GFP::Z...
Another example of a metaphase-arrested oocyte expressing mCherry::tubulin (top), dissected into Mei...
Shows a metaphase-arrested oocyte expressing degron::GFP::ZYG-9, GFP::ASPM-1, and mCherry::tubulin. ...
Shows a metaphase-arrested klp-18(RNAi) oocyte expressing mCherry::tubulin (left) and degron::GFP::Z...
(A) Movie stills from Metaphase I-arrested (emb-30(RNAi)) oocytes expressing mCherry::tubulin and de...
Shows a metaphase-arrested klp-18(RNAi) oocyte expressing GFP::ASPM-1, mCherry::tubulin (left) and d...
(A) Movie stills from Metaphase I-arrested klp-18(RNAi)) oocytes expressing mCherry::tubulin and deg...
Another example of a metaphase-arrested oocyte expressing degron::GFP::ZYG-9, GFP::ASPM-1, and mCher...
(A, B) Spindles from Metaphase I-arrested (emb-30(RNAi)) oocytes (A) or unarrested oocytes (B) treat...
Shows a Metaphase II oocyte spindle labeled with degron::GFP::ZYG-9 and mCherry::histone; the polar ...
Shows a Metaphase II oocyte spindle labeled with degron::GFP::ZYG-9 and mCherry::histone; the polar ...
Oocytes of most species assemble and maintain a functional bipolar spindle in the absence of centro...
Shows a metaphase-arrested klp-18(RNAi) oocyte expressing GFP::ASPM-1, mCherry::tubulin (left) and d...
(A-D) Time-lapse maximum intensity projection images of live control and TS mutant oocytes expressin...
(A) IF imaging of oocyte spindles in the ZYG-9 AID strain in either control or tac-1(RNAi) condition...
Shows a metaphase-arrested klp-18(RNAi) oocyte expressing mCherry::tubulin (left) and degron::GFP::Z...
Another example of a metaphase-arrested oocyte expressing mCherry::tubulin (top), dissected into Mei...
Shows a metaphase-arrested oocyte expressing degron::GFP::ZYG-9, GFP::ASPM-1, and mCherry::tubulin. ...
Shows a metaphase-arrested klp-18(RNAi) oocyte expressing mCherry::tubulin (left) and degron::GFP::Z...
(A) Movie stills from Metaphase I-arrested (emb-30(RNAi)) oocytes expressing mCherry::tubulin and de...
Shows a metaphase-arrested klp-18(RNAi) oocyte expressing GFP::ASPM-1, mCherry::tubulin (left) and d...
(A) Movie stills from Metaphase I-arrested klp-18(RNAi)) oocytes expressing mCherry::tubulin and deg...
Another example of a metaphase-arrested oocyte expressing degron::GFP::ZYG-9, GFP::ASPM-1, and mCher...
(A, B) Spindles from Metaphase I-arrested (emb-30(RNAi)) oocytes (A) or unarrested oocytes (B) treat...
Shows a Metaphase II oocyte spindle labeled with degron::GFP::ZYG-9 and mCherry::histone; the polar ...
Shows a Metaphase II oocyte spindle labeled with degron::GFP::ZYG-9 and mCherry::histone; the polar ...
Oocytes of most species assemble and maintain a functional bipolar spindle in the absence of centro...
Shows a metaphase-arrested klp-18(RNAi) oocyte expressing GFP::ASPM-1, mCherry::tubulin (left) and d...
(A-D) Time-lapse maximum intensity projection images of live control and TS mutant oocytes expressin...
(A) IF imaging of oocyte spindles in the ZYG-9 AID strain in either control or tac-1(RNAi) condition...