Timelapse of stable bipolar spindle expressing GFP::ASPM-1 and mCherry::tubulin.

Shows a metaphase-arrested oocyte expressing degron::GFP::ZYG-9, GFP::ASPM-1, and mCherry::tubulin. On the left is a merge of ASPM-1 (green) and tubulin (magenta), and on the right is ASPM-1 alone (gray). When dissected into a control Meiosis Media solution, no major changes in spindle morphology were observed. Corresponds to Fig 2B. For the duration of the timelapse, ASPM-1 foci remained focused and stable at either end of the spindle. A faint population of GFP::ZYG-9 can be observed on spindle microtubules. Time elapsed shown in (min):(sec). Scale bar = 5μm. (MOV)