Additional file 8 of FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex
- Martyna Sochacka (14149008)
- Radoslaw Karelus (14149011)
- Lukasz Opalinski (14149014)
- Daniel Krowarsch (14149017)
- Martyna Biadun (14149020)
- Jacek Otlewski (14149023)
- Malgorzata Zakrzewska (14149026)
Publication date
November 2022
DOIAbstract
Additional file 7: Fig. S6. Effect of NOLC1 and TCOF1 knock-down on the nucleolar localization of FGF12 and its interaction with NOLC1 and TCOF1. HEK 293 cells were transfected with NOLC1/TCOF1-targeting siRNA or scramble siRNA (control). Fluorescence images of in situ PLA using a mixture of rabbit anti-FGF12 and mouse anti-NOLC1 or anti-TCOF1 antibodies. Cell nuclei were labeled with NucBlue Live and cells were analyzed with fluorescence microscopy. The dashed line indicates the cell area. The scale bar represents 20 μm
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