The effect of the loss of FLA3 on the location of proteins associated with the flagellum and flagellar attachment zone.

<p>Bloodstream form FLA3 RNAi cells were grown under induced or noninduced conditions for 24 h. Panel A. Immunolocalization of α-tubulin and paraflagellar rod protein 2 (PFR2) in induced and noninduced cells. The upper panels represent the fluorescence image showing location of the protein (red) and nucleus/kinetoplast (blue), while the lower panels represent the corresponding phase image. Bar = 10 µm. Panel B. A sample section through a detached flagellum. Bar = 500 nm Panel C. Localization of FAZ1 in induced and noninduced cells. The upper panels represent the fluorescence image showing location of the protein (red) and nucleus/kinetoplast (blue), while the lower panels represent the corresponding phase image. Bar = 10 µm. The arrowhead indicates apparent aberrant accumulation of FAZ1 at the posterior end of an induced cell which has a single attachment zone containing FAZ1 associated with the attached flagellum (arrow in phase and fluorescence image).