Detection of W22703 and its derivatives in the hemolymph of <i>G</i>. <i>mellonella</i>.

All photos show the hemolymph area of G. mellonella. (A) G. mellonella tissue sections 24 h p.i. with W22703 and its mutants. Staining was conducted using the anti-Yersinia antibody. (B) Tissue sections of larvae 24 h p.i. with W22703 tcaA::rfp, W22703 ΔHE tcaA::rfp, W22703 PHE::rfp, and W22703 ΔHE tcaA::rfp/pACYC HE. Staining was performed with the Yersinia-specific antibody (top row) or the anti-RFP antibody (bottom row), which was applied to the same tissue sections, to detect TcaA production as indicated exemplarily by arrows. Preparations of ten infected animals per strain were carried out. Photos of representative preparations are shown; the scales are indicated. 1 = exoskeleton, 2 = musculature, 3 = fat tissue, 4 = hemolymph.