Induction of <i>cit</i> promoters in <i>G</i>. <i>mellonella</i>.

(A) Scheme of citrate metabolism genes and fluorescent reporter plasmid pTLGR-Pcit used for microscopy. Fluorescence microscopy at different time points of E. faecalis JH2-2 cells harboring pTLGR-Pcit plasmid grown in LB and LB with 0.5% citrate (LBC), scale bar 5 μm (B); or G. mellonella hemolymph extracted at different time points after E. faecalis JH2-2 pTLGR-Pcit infection, scale bar 50 μm (C). Representative individuals of inoculated larvae are shown in (C). Two independent experiments were carried out and representative images acquired are shown.