Identification of markers highly expressed in hiPSCs compared to NPCs derived from a clinical-grade iPSC stock line.

(A) TPM values of MIR302CHG, CUZD1, LIN28A, ESRG, CNMD, SFRP2 and TBP in hiPSCs and NPCs show the high absolute expression of MIR302CHG. Data are represented as the mean ± SEM (n = 3). (B) TPM values of three MIR302CHG splice variants, ENST00000510655.1, ENST00000509938.1 and ENST00000505215.1, in hiPSCs and NPCs. Data are represented as the mean ± SEM (n = 3). (C, D) Scatter plots of the TBP-normalized gene expression of MIR302CHG, CUZD1 and POU5F1 by qRT-PCR for mixtures of hiPSCs and NPCs (C) or day 4 cells (D). The dots and lines in the center of the scatter plots indicate the experimental data and mean values of the data, respectively. UD: undetermined; D4C: day 4 cell. *p p <0.01 by paired Student’s t-tests against iPSCs with the Bonferroni correction (A, B) and by Tukey-Kramer post hoc tests against the sample with 0% iPSCs (C, D). NS, not significant.