Characterization of hiPSCs.
- Fumitoshi Tsurumi (6470369)
- Shiro Baba (6470372)
- Daisuke Yoshinaga (6470375)
- Katsutsugu Umeda (113467)
- Takuya Hirata (1604398)
- Junko Takita (6470378)
- Toshio Heike (113471)
Publication date
March 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Immunostaining of the pluripotency markers NANOG (green) and TRA-1-81 (red) in hiPSCs. Nuclei were counterstained with Hoechst 33342 (blue). Scale bar: 20 μm. (B) Karyotyping of hiPSCs. (C) Quantitative PCR analysis of total and exogenous Oct3/4, Sox2, Klf4, and c-Myc expression in hiPSCs. Black and white bars indicate total and exogenous gene expression, respectively. ESC: human embryonic stem cell, NC: negative control (water).</p
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