Increase of one-to-one particle encapsulation yield using dielectrophoretic alignment technique with boxcar-type electrodes

We developed a technique which can increase the yield of one-to-one particle encapsulation by applying the dielectrophoretic particle alignment technique using boxcar-type electrodes. Dielectrophoretic force generated by the boxcar-type electrodes accelerate and decelerate the particles periodically as they flow in the electrode region. Further, the dielectrophoretic force is turned on and off at constant frequency. The force exerted on the particle periodically over space and time can align them in the streamwise direction with even interval. In this study, the boxcar-type electrodes were installed in the microchannel in the region upstream of the flow-focusing channel in which the water-in-oil droplets were generated. By adjusting the on-off period of the applied voltage generating the dielectrophoretic force to the period of the droplet generation, each particle could be separately encapsulated in the droplets. The principle of particle alignment using periodic force was first described based on a one-dimensional model. The flow structure and the characteristics of the droplet generation in the flow-focusing channel was then discussed in relation to the surface tension of the fluids and the wettability of the wall. We measured the velocity distribution of the particles flowing in the boxcar electrode region to evaluate the effects of the droplet generation on the motion of the particles and the alignment performance. The results showed that the particle could be aligned in the fluctuating flow caused by the droplet generation, and each particle can be encapsulated in different droplets. This was further demonstrated by measuring the probability function of the droplets containing specific number of particles, which showed that 100% yield of one-to-one particle encapsulation can be achieved under the investigated condition of particle number density of 0.4. Moreover, the throughput increased 46% compared to the case of having the particles supplied randomly