Comparison and quality analysis of total RNA extraction methods from different tissues of Pyrus betulaefolia Bunge

Objective: Aiming at the tissues of Pyrus betulaefolia Bunge are abundant in amylose, hydroxybenzene and other secondary metabolities, the study screened the method in order to obtain highquality RNA. Methods:The different tissues(root, stem, leaf, flower and fruit) were used as the materials, and the total RNA were extracted by Trizol method, the improved CTAB method and RNAprep Pure Plant Kit method. The concentration and purity of the extracted RNA were analyzed by Agarose Gel Electrophoresis and NanoDrop 2000. Results: The total RNA can be extracted by the 3 methods, and the bands of the total RNA extracted by RNAprep Pure Plant Kit method were clear. The purities were 2.07, 2.02, 2.01, 2.01 and 2.02 respectively, and the concentrations were 186.2 ng · μL- 1, 174.5 ng · μL- 1, 163.6 ng · μL- 1, 167.3 ng · μL- 1 and 121.1 ng · μL- 1 respectively. Additionally, the total RNA extracted by RNAprep Pure Plant Kit method could be used for reverse transcription, and the amplification bands of PbUBI gene were specific. Conclusion: The RNAprep Pure Plant Kit method was suitable for different tissues of Pyrus betulaefolia Bunge and laid the foundation for the further molecular biology research