In the present paper, Differential Evolution algorithm is used to perform structural identification of mass and stiffness properties of civil structures from dynamic test results. Identification is performed initially starting from exact values of modal parameters (frequencies and mode shapes). Robustness of the algorithm is then tested by adopting pseudo-experimental input data, obtained by adding to exact data some statistic scattering, representing experimental measurement error. Different objective functions are adopted in identification procedure, and results are compared with those obtained adopting classical gradient method. The method is used to identify masses, elastic moduli and stiffnesses of external constraints of a RC frame st...