We consider a linear full elliptic second order partial differential equation in a d-dimensional domain, d 65 1, approximated by isogeometric collocation methods based on uniform (tensor-product) B-splines of degrees p := (p1, ... , pd), pj 65 2, j = 1, ... , d. We give a construction of the inherently non-symmetric matrices arising from this approximation technique and we perform an analysis of their spectral properties. In particular, we find and study the associated (spectral) symbol, that is, the function describing their asymptotic spectral distribution (in theWeyl sense) when the matrix-size tends to infinity or, equivalently, the fineness parameters tend to zero. The symbol is a non-negative function with a unique zero of order two...