Sars-cov-2 serum neutralization assay: A traditional tool for a brand-new virus
- Matusali G.
- Colavita F.
- Lapa D.
- Meschi S.
- Bordi L.
- Piselli P.
- Gagliardini R.
- Corpolongo A.
- Nicastri E.
- Antinori A.
- Ippolito G.
- Capobianchi M. R.
- Castilletti C.
- Abbate I.
- Agrati C.
- Aleo L.
- Alonzi T.
- Amendola A.
- Apollonio C.
- Arduini N.
- Bartolini B.
- Berno G.
- Biancone S.
- Bibbo A.
- Brega C.
- Canali M.
- Cannas A.
- Capobianchi M. R.
- Carletti F.
- Carrara S.
- Casetti R.
- Castillettiy C.
- Chiappini R.
- Ciafrone L.
- Cimini E.
- Coen S.
- Condello R.
- Coppola A.
- D'arezzo S.
- Di Caro A.
- Di Filippo S.
- De Giuli C.
- Fabeni L.
- Felici L.
- Ferraioli V.
- Forbici F.
- Garbuglia A. R.
- Giombini E.
- Gruber C. E. M.
- Khouri D.
- Lalle E.
- Leone B.
- Matusali G.
- Mazzarelli A.
- Messina F.
- Minosse C.
- Montaldo C.
- Neri S.
- Nisii C.
- Petrivelli E.
- Petroni F.
- Petruccioli E.
- Pisciotta M.
- Pizzi D.
- Prota G.
- Rozera G.
- Rueca M.
- Sabatini R.
- Sarti S.
- Sberna G.
- Sciamanna R.
- Selleri M.
- Selvaggi C.
- Stellitano C.
- Toffoletti A.
- Truffa S.
- Turchi F.
- Valli M. B.
- Venditti C.
- Vincenti D.
- Vulcano A.
- Zambelli E.
- Bevilacqua N.
- Bordoni V.
- D'abramo A.
- Ippolito G.
- Lepore L.
- Mariano A.
- Palazzolo C.
- Lorenzini P.
- Bordoni V.
- Notari S.
- Sacchi A.
- Scorzolini L.
- Bettini A.
- Francalancia M.
- Specchiarello E.
- Federica M.
- Gaetano D.
- Luigi F.
- Barbara G.
- Roberto I.
- Giovanni M.
- Mirco M.
- Rachele S.
DOIAbstract
SARS-CoV-2 serum neutralization assay represents the gold standard for assessing antibody-mediated protection in naturally infected and vaccinated individuals. In the present study, 662 serum samples collected from February 2020 to January 2021 from acute and convalescent COVID-19 patients were tested to determine neutralizing antibody (NAb) titers using a microneutralization test (MNT) for live SARS-CoV-2. Moreover, anti-SARS-CoV-2 IgG, IgA, and IgM directed against different viral antigens were measured by high-throughput automated platforms. We observed higher levels of NAbs in elderly (>60 years old) individuals and in patients presenting acute respiratory distress syndrome. SARS-CoV-2 NAbs develop as soon as five days from symptom o...
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