Isolation of a minimal <i>sna-rg</i> enhancer.
- Mar García-Ferrés (13899772)
- Carlos Sánchez-Higueras (13899775)
- Jose Manuel Espinosa-Vázquez (13899778)
- James C-G Hombría (13899781)
Publication date
October 2022
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Scheme of DNA elements tested indicating the location of the STAT binding sites. The sna-rg R2 reporter comprises the cis-regulatory sequence deleted in the CRISPR-Cas9 snaΔrgR2 mutation. Expression at st11 of the full sna-rg-GFP (B), sna-rg R1-GFP (C), sna-rg R2-GFP (D), and sna-rg R2P2-GFP (E) constructs. (F) Scheme showing the fragment deleted in the snaΔrgR2 mutation indicating the sequence of the sgRNAs used and the sequences flanking the deletion. (TIF)</p
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