Predicted genomic and protein effects of the mutant <i>snai2</i> alleles.

A schematic of the snai2 gene displays where the three guide RNAs (gRNAs) were designed for CRISPR/Cas9 directed mutagenesis (red arrowheads) as well as the single nucleotide polymorphism (SNP) location in the mutagenesis derived snai2sa24539 allele (red *) (A). The endogenous stop codon is indicated by the red line in exon 3. Predicted effects on the amino acid sequence (B) and protein structure (C) of wild-type, snai2112Δ, and snai2sa24539 are depicted. For the snai2112Δ allele, a 112 bp deletion within the beginning of exon 2 leads to a truncation prior to the zinc-finger domain. Italicism of the amino acid sequence and the grey region of the structure indicate a region of missense amino acids prior to the early stop codon. The snai2sa24539 allele SNP is a stop codon.