Immuno-profiling of host serum with a <i>Trypanosoma vivax</i>-specific antigen array.

(A) Fluorescence observed after application of host sera to a customised array of 600 peptides representing 63 T. vivax-specific antigens. From top to bottom, the panels show responses to serum from three locations (Brazil, Cameroon and Kenya respectively), in addition to two negative controls (serum from UK cattle and from Cameroonian cattle that tested T. vivax-negative but T. congolense-positive). Control peptides are shown in boxes at top left and bottom right of each array. The top row of the array (boxed) contains peptides exclusively derived from vivaxin proteins. (B) Normalized intensity values of immuno-fluorescent responses in (A) are plotted for all array peptides, arranged by gene family. Individual gene families are indicated by alternating white and pink shading. Strongly-responding peptides belonging to vivaxin (Fam34) are indicated by blue shading.