Chromatin features of <i>WT</i> and <i>sir2</i> strains at the telomeres.

(A) Illustration of 3000 bp from the left telomere of chromosome 3 in the Integrative Genome Viewer (IGV). Shown are tracks generated from ChIP-seq data for Sir2 (dark green) and H4K16 acetylation (light green), followed by MNase-seq tracks of WT (grey) and sir2 (orange) strains and the DANPOS-calculated difference between sir2 and WT (black). FDR value is given under/adjacent to the nucleosome with the largest difference. Reb1 consensus sites are denoted in blue and Reb1 occupancy levels (ChIP-exo) are shown in dark red. Genes are shown in the bottom track in grey. (B) Same as in (A), except showing a 3000 bp region from the right arm of chromosome 4. Note that YDR544C is a dubious ORF, unlikely to encode a protein. (C) MNase-seq read counts of the summit of the nucleosomes covering the STR-D (circles) or X-core loci (squares) of 22 telomeres in WT (grey) and sir2 (orange) strains. (D) Quantification of both acetylated H4K16 and Sir2 occupancy at the STR-D locus compared to the X-core region and euchromatin presented as box-plots. 15 STR-D loci were compared to 15 X-core loci and 24 randomly selected euchromatic nucleosomes.