Reb1 binds to subtelomeres and destabilizes nucleosomes.

(A) Meta-X-element analysis of 7 left and 5 right telomere ends. The top panel shows the normalized MNase-seq signal of WT (grey) and sir2 (orange) centered on the summit of the nucleosome with the highest occupancy change. In the middle panel, Reb1 consensus sites (X) and occupancy (dots) presented as a heat map are shown for the individual X-elements. (B) MNase-qPCR of WT and sir2 strains carrying a deletion of the endogenous REB1 gene and expressing either a WT or mutant temperature-sensitive plasmid-borne allele of REB1. Strains were grown at restrictive temperature. (C) Chromatin immunoprecipitation followed by qPCR of the indicated strains. Ct values were first normalized to ACT1, followed by normalization to input. All data are shown as mean ± SEM (n = 3, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns = not significant).