Pirk-like is a feedback inhibitor of IMD pathway.

(A) Density plot showing the effect of Pirk-like knockout on IMRG expression after E. cloacae or PBS treatment. Gray shaded area indicates values used to define differentially expressed genes or resistant genes. (B) Venn diagram showing the core genes enriched among the differentially expressed IMRG shared by different treatment groups. (C-E) Volcano plots showing IMRG expression of treatment groups compared with control (WT_PBS 12 h). Each gene is marked as a dot; the red plots represent significantly enriched genes. The blue plots represent downregulated genes. The broken lines indicate the marginal lines selecting DEGs, with the horizontal broken lines denoting the p value threshold (p Pirk-like in iEGFP and iRel2 mosquitoes either uninfected or infected with E. cloacae for 12 h. Data are shown as mean ± SEM. ****p -/- mosquitoes were stimulated for 12 h with OD600 = 1 E. cloacae or PBS at PE stage and then analyzed using qRT-PCR for CecB, CecG, DefC, DefD and GAM transcriptions. Data are shown as mean ± SEM. ****p (TIF)