Silencing of EcR increases susceptibility to infection and induces IMRG expression.

(A) Survival of EcR knockdown (iEcR) or control (iEGFP) mosquitoes after E. cloacae infection. n = 3 cohorts (total 90 mosquitoes). P values for differences in survival times between iEGFP and iEcR mosquitoes were determined by means of the log-rank test. (B) Density plot showing the effect of EcR silencing (iEcR) on IMRG expression after E. cloacae or PBS treatment. Gray shaded area indicates values used to define differentially expressed genes or resistant genes. (C) Venn diagram and UpSet plot representing unique and shared IMRGs. The overlapping regions represent gene cohorts that are concomitantly regulated by one or two experimental conditions. (D) Hierarchical cluster analysis of IMRGs in (C) with log2(FPKM) values. Gene names are shown on the right. Data are from three biological replicates. Pearson correlation >0.97 for all replicates.