Additional file 1 of Intestinal microbiome-mediated resistance against vibriosis for Cynoglossus semilaevis

Additional file 1: Supplementary Figure 1. The alpha diversity of ARG Type in the intestinal microbiome of C. semilaevis. Except for Simpson, the diversity measured by ACE, Chao 1, good_coverage, observed species, Shannon, and was higher in the resistant family compared with susceptible family, but not significantly between two families. Supplementary Figure 2. The family-specific KEGG enrichment pathways of microbial genes. Circles and triangles represent the resistant family-specific and susceptible family-specific KEGG enrichment pathways, respectively. The color indicates the enrichment level (adjusted p < 0.05), the more enrichment of the pathway, the smaller the p value. The size of circles and triangles was dependent on the gene ratio. Supplementary Figure 3. The locations of Phaeobacter genes involved in lipid metabolism and immune pathways. Here we have used the assembled bins assigned to Phaeobacter for locating Phaeobacter genes. One bold arrow represents a KO gene, and its length indicates the length of the gene. The color of gene name represents the KO pathway of this gene involved. Supplementary Figure 4. Intestinal microbial, host functional, and combinatorial markers for discriminating the resistant family from the susceptible family in C. semilaevis. The accuracy, F1 score, and AUC were used for evaluating the performance of all microbes (yellow curve), top 4 important microbes (blue curve), top 3 important microbes (orange curve), 11 ko genes (the microbiome-associated DEGs significantly enriched pathways; green curve), top 5 important ko genes (pink curve) of 11 ko genes, top 5 important ko genes (wathet curve) of 11 ko genes, and the combination of top 4 important microbes and 11 ko genes (red curves). Supplementary Table 1. Detailed sample information of the Cynoglossus semilaevis samples collected from resistant and susceptible families. Supplementary Table 2. The detailed information of top 20 KEGG enrichment pathways (adjusted p < 0.05) for un- the down-expressed genes in the resistant family, respectively. Supplementary Table 3. The KEGG pathway of 8 differentially expressed genes between the resistant and susceptible families. Supplementary Table 4. The locations of Phaeobacter genes involved in lipid metabolism and immune pathways