Effects of B1, B2, B3, B4, V1, and V2 cell lysis methods on BCMC1 taxonomy.

DNA extractions were performed on BCMC1 using the MagMAX Total nucleic acid kit. Each bar represents the average value of three replicates. Significant differences (DESeq2 adjusted p 2 fold change > 1.5) from the expected value are indicated by the presence of asterisks. Exp stands for expected, B1 (bead beat twice for 1 min at 6.5 m/sec with 1 min interval on ice), B2 (bead beat for 1 min at 6.5 m/sec), B3 (bead beat twice for 1 min at 3.5 m/sec with 1 min interval on ice), B4 (bead beat at 3.5 m/sec for 60 sec), V1 (vortex at 1800 rpm for 15 min), V2 (vortex at 1800 rpm for 10 min), and C1 (incubation at 80°C for 20 min and 37°C for 60 min).