Effect of plasmid DNA conformation on DNA measurement methods.

<p>(A) Comparison of various methods on supercoiled plasmid DNA quantification. (B) Hoechst dye-based DNA quantification method. (C) Quant-iT dsDNA BR quantification assay. (D) OD₂₆₀ DNA quantification method. S-LB: supercoiled plasmid treated with SspI reaction buffer. S-NB: supercoiled plasmid treated with Nt.BspQI reaction buffer. S-CB: supercoiled plasmid treated with topoisomerase I reaction buffer. a, b, c, d and e : groups with significant difference to each other (Duncan's multiple range test, P<0.05).