The Hth interactor Exd, is expressed in the IPCs and is equally required for <i>dilp</i> expression.

(A-B) Antibodies against Exd detect expression in L3 and adult IPC nuclei, marked by dilp2-GAL4R driven UAS-CD8GFP expression. (C-C’) Dilp2, (E-E’) Dilp3 and Dilp5 (G-G’) protein levels are reduced in adult IPCs upon Exd depletion in the IPCs compared to balanced age-controlled siblings. Insets (C’, E’, G’) show Dilp2, -3 and -5 only (grayscale). Brains from knockdown and balanced siblings were dissected and stained in the same tube. Expression of CD8-GFP was used to distinguish control and test genotypes, and to label the IPCs. Knockdown of exd in the IPCs reduced expression of (D) dilp2 and (E) dilp3 significantly while dilp5 is upregulated. Flies were reared at controlled densities. RNA was collected from 10–15 female fly heads. After normalization, dilp expression was normalized to a control dilp2-GAL4R; btlGAL80 driving UAS-mCherryRNAi.