Hth and Exd have differing effects on Dilp protein and mRNA levels in late L3.

(A-A’, B-B’) representative images showing reduced Dilp2 and Dilp5 protein levels in late L3 (100h after egg laying) larvae genetically depleted of hth compared to balanced sibling controls (CyO, left panels). (C-C’, D-D’) IPC-specific exd knockdown results in decreased Dilp2 and unaltered Dilp5 protein levels in IPC somata. (E) dilp2 mRNA levels are significantly reduced upon hth depletion and not significantly reduced upon exd depletion. (F) dilp5 mRNA levels are severely reduced hth knockdown larvae and are unaltered in exd knockdown. (ns, not significant, ***p<0.001, ****p<0.0001), one-way ANOVA with post-hoc Dunnett’s test to correct for multiple comparisons).