Validation of the <i>Chlamydia muridarum incS</i> conditional mutant.

(A) Schematic representation of the incSCm locus of wild-type (WT) and incSCm::bla mutant C. muridarum strains, and of the primers used for mutant validation by PCR. P1 (TC0424 Up Fw), P2 (TC0424 (691–719)). (B) DNA gels of PCR products generated using the following combination of genomic DNA template/primer, as described in (A). WT/P1P2 (lane 2) and incSCm::bla/P1P2 (lane 3). The ladder is shown in lane 1. (C) The site of insertion of the group II intron was confirmed by Sanger sequencing. A translation of the resulting IncSCm truncated peptide is presented. The asterisk denotes the early stop codon introduced by the insertion of the group II intron (brown: IncSCm, blue: group II intron). (TIF)