Analysis of genes regulated by hemin vs. genes regulated by Hap1.

(A) GO analysis of genes induced or repressed at least 2x in all comparisons showed. Only the top term in the GO hierarchy is shown. Full lists are available in S4 and S5 Tables. (B) Venn diagram of overlap of genes induced at least twice. The four groups are: genes induced at least 2-fold in HAP1 vs. hap1-/- in the presence of hemin (violet) or in regular medium (yellow) and the genes induced at least 2-fold by hemin in the HAP1 strain (green) or in the hap1-/- strain (pink orange). The boxes on the left give the names of the seven genes induced by hemin and by HAP1 with or without hemin (dark green), and by hemin and by HAP1 in the presence of hemin only (turquoise). CFEM hemophores and ferric reductase-like genes FRP1, FRP2 are in bold. The box on the right lists the enriched GO categories of genes overexpressed in the presence of hemin in both HAP1 and hap1-/- cells. The full lists of the genes in each of the populated categories are shown in supplementary S2 Table.