Figure 3 components

Figure 3. Response of enzyme-functionalized GFETs to two nitriles. Figure 3a. Normalized response of wild-type QueF and QueF-Y5Y fusion protein to concentrations of preQ0 to 500nM. Gray vertical lines represent approximate timings for analyte addition. Arrowed symbols: ● = sensor wash in phosphate buffer, ◆ = addition of 4 µM NADPH, ■ = addition of 1 M substrate. GFET conditions: Vg: 500 mV, Vd: 5 mV. Figure 3a. Normalized response of wild-type QueF and QueF-Y5Y fusion protein to concentrations benzyl cyanide (BnCN) to 500nM. Gray vertical lines represent approximate timings for analyte addition. Gray vertical lines represent approximate timings for analyte addition. Arrowed symbols: ● = sensor wash in phosphate buffer, ◆ = addition of 4 µM NADPH, ■ = addition of 1 M substrate. GFET conditions: Vg: 500 mV, Vd: 5 mV. Figure 3c. Normalized response curve fittings for each sample. Error bars ranges are mean ± standard deviation. Number of replicates are given in Table 1. Standard deviation in panel c is calculated from the pooled variance of all signals over 1 min.