We investigate the behavior of eigenvalues for a magnetic Aharonov-Bohm operator with half-integer circulation and Dirichlet boundary conditions in a planar domain. We provide sharp asymptotics for eigenvalues as the pole is moving in the interior of the domain, approaching a zero of an eigenfunction of the limiting problem along a nodal line. As a consequence, we verify theoretically some conjectures arising from numerical evidences in preexisting literature. The proof relies on an Almgren-type monotonicity argument for magnetic operators together with a sharp blow-up analysis