Microfabrication of LOC Device Utilizing Micro Pump and Micro Valve Flow Mechanism

Over the last decade microfabrication techniques have been implemented for the fabrication of Lab on a-chip (LOC) systems. These systems can conduct several types of chemical and cellular analysis, facilitate fluid transport, combine, or separate liquid samples. The LOC systems incorporate microarray and microfluidic systems to assemble a micro total analytic system. The miniaturization of analytical systems that integrate microfluidic LOCs (lab on a chips) with the application of sensors is crucial to automation of laboratory procedures. This will allow reduction of the time elapsed to conduct chemical and biological tests, reducing manufacturing costs and increasing production capabilities. This study will focus on the development and fabrication of an LOC system that contains micro pump and micro valve devices to precisely actuate a series of reagents, which by selectively pressurize the polydimethylsiloxane (PDMS) channels will actuate multiple reagents in a controlled manner. The PDMS will be pressurized by a roller bar that is operated by a programmable microprocessor. These sub-cellular biomaterial micropatterns can effectively be employed to study various engineering applications