Kathepsine C:Een allosterisch enzyme

In chapter I an introduction into allosteric systems is given. In chapter II is a detailed method is described for the applica of Gly-Phe--p. nitroanilide (GPNA) as a substrate for the activity assay of the lysosomal enzyme cathepsin C. It is an allosteric which is activated by Cl-, Br-, 1-, CNS-, NO-3 and ClO3 ions. (Chapter III). At high activator concentrations the enzyme Michaelis-Menten kinetics, at low concentrations there is substrate cooperativity. Thus, in the absence of added activators a small amount of the substrate Ala-Phe-amide enhances the hydrolysis rate of a low concentration of GPKA; at higher concentrations it is a competitive inhibitor........