Objective: To investigate the construct validity and test-retest reliability of the Walking Questionnaire, a patient-reported measure of activity limitations in walking in people with a lower limb amputation.Design: Cross-sectional study.Setting: Outpatient department of a rehabilitation center.Participants: People with a lower limb amputation (N = 172; mean age +/- SD, 65 +/- 12y; 71% men; 82% vascular cause) participated in the study, 33 of whom also participated in the reliability study.Interventions: Not applicable.Main Outcome Measures: Construct validity was investigated by testing 11 hypotheses: limitations in walking according to the Walking Questionnaire will be greater in people with a lower limb amputation who (1) are older, (2) ...